Cellular Electrophoretic Mobility and the Mitotic Cycle
نویسندگان
چکیده
منابع مشابه
Cellular Electrophoretic Mobility and the Mitotic Cycle
The electrophoretic mobility of RPMI No. 41 cells grown in suspension, parasynchronized by double thymidine blocking and cold shock, is reported. No. 41 cells have a higher electrophoretic mobility during the mitotic peak phase than at other times in the mitotic cycle. Treatment of parasynchronous cells by neuraminidase reduces the mobility to the same value irrespective of the stage of the cel...
متن کامل314 Electrophoretic Mobility and Agglutinability
I t has been recognized since 1954 (1) that the red cells of mice of some strains, notably A, can be specifically agglutinated more readily by anti-H-2 sera than those of others. This difference has been shown to apply to several 1t-2 antigens (2) and has been ascribed to a different cell concentration of antigenic determinants (3). Experience shows, however, that nonspecific agglutinability is...
متن کاملColcemid and the mitotic cycle.
The precise segregation of replicated chromosomes to daughter cells during mitosis depends on the formation of a bipolar spindle composed primarily of microtubules (MTs). Since MTs are highly dynamic structures whose spatial organization is critical for proper spindle function, physical and chemical agents that interfere with MT behavior invariably disrupt mitosis. Perhaps the most notable of t...
متن کاملElectrophoretic Mobility Shift Assay (EMSA).
Transcriptional regulation of gene expression is controlled through the binding of sequence-specific DNA-binding proteins (transcription factors) to the regulatory regions of genes. The exact gene expression program of a cell is determined by the spectrum of transcription factors present with the nucleus of a cell. The presence of these factors is dependent upon the cell type being examined and...
متن کاملThe macrophage electrophoretic mobility test for malignancy.
Lymphocytes obtained from the blood of patients suffering from malignant disease liberate, when incubated with a basic protein obtained from carci nomatous tissue or the brain, a substance ( slowing factor ) which reduces the electrophoretic mobility of guinea pig macrophages. Lymphocytes from normal subjects or patients with non malignant tumors do not liberate this slowing factor. The macrop...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1966
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.49.4.717